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Wednesday, April 15, 2020 | History

2 edition of Optical and electron microscopy studies of the retention of asbestos fibers in rat lung found in the catalog.

Optical and electron microscopy studies of the retention of asbestos fibers in rat lung

F. L Ball

Optical and electron microscopy studies of the retention of asbestos fibers in rat lung

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  • 29 Currently reading

Published by Dept. of Energy, Oak Ridge Nationa Laboratory, for sale by the National Technical Information Service] in Oak Ridge, Tenn, [Springfield, Va .
Written in English

    Subjects:
  • Rats,
  • Lungs,
  • Asbestos fibers

  • Edition Notes

    StatementF. L. Ball, W. D. Gude, Biology Division, and A. J. Weinberger
    SeriesORNL/TM ; 6827
    ContributionsGude, William D., joint author, Weinberger, A. J. joint author, Oak Ridge National Laboratory. Biology Division
    The Physical Object
    Paginationv, 13 p. :
    Number of Pages13
    ID Numbers
    Open LibraryOL14881860M

    Measurement of asbestos bodies using light microscopy underestimates the total lung fiber burden by a significant percentage. The total number of asbestos bodies in digested lung is usually 10 to 10, times less as compared to the total number of uncoated fibers detected by electron microscopy. One of the open questions regarding the asbestos problem is the fate of the mineral fibres in the body once inhaled and deposited in the deep respiratory system. In this context, the present paper reports the results of an electron microscopy study of both mineral fibres and asbestos bodies found in the lung tissue of a patient who died of malignant mesothelioma due to past Author: Dario Di Giuseppe, Alessandro Zoboli, Ruggero Vigliaturo, Reto Gieré, Maria Paola Bonasoni, Orietta.   The fibrogenicity and carcinogenicity of asbestos fibers are dependent on several fiber parameters including fiber dimensions. Based on the WHO (World Health Organization) definition, the current regulations focalise on long asbestos fibers (LAF) (Length: L ≥ 5 μm, Diameter: D 3). However air samples contain short asbestos fibers Cited by: Asbestos analysis, scanning electron microscope, SEM, SEM-EDX. We analyse your samples for hazardous materials like asbestos or man made mineral fibres by means of high-resolution scanning electron microscopes according to relevant .


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Optical and electron microscopy studies of the retention of asbestos fibers in rat lung by F. L Ball Download PDF EPUB FB2

Get this from a library. Optical and electron microscopy studies of the retention of asbestos fibers in rat lung. [F L Ball; William D Gude; A J Weinberger; Oak. Authors: Fish, B.R. Publication Date: Mon Jul 01 EDT Research Org.: Oak Ridge National Lab., Tenn. (USA) OSTI Identifier: Report Number(s).

A combination of light and scanning electron microscopy was used in the evaluation of the retention of particles in rat lungs after intratracheal instillations of solutions containing asbestos.

Individual lobes of the lung were prepared for light and electron optical studies. Asbestos fibers appeared as fibrils and were usually associated with. Asbestos Fibers. When electron microscopy was applied to asbestos analysis, hundreds of fibers were discovered present too small to be visible in any light microscope.

There are two different types of electron microscope used for asbestos analysis: Scanning Electron Microscope (SEM) and Transmission Electron Microscope (TEM). There are no worker mortality studies available that relate the risk of asbestos-related health effects to exposures as measured by TEM.

OSHA acknowledges, as Mr. Feuerstein pointed out, that PCM does not count all of the asbestos fibers that may be present in the air since fibers thinner than about microns cannot be seen using this method.

The case reported herein was of an individual who died from a pleural mesothelioma. Paraffin-embedded lung tissue was examined by a laboratory using scanning electron microscopy (SEM) and was reported to contain elevated asbestos body concentrations and five fibers, of which two were asbestos (one chrysotile and one tremolite).Cited by: Length distributions of amosite fibers aerosolized, but before inhalation versus after retention in the lung were similar, whether determined by ashing/SEM or in situ CLSM.

In contrast, the fraction of short MMVF10a and MMVF33 fibers increased and the geometric mean fiber lengths of both MMVFs decreased by approximately 60% during by: Asbestos Fibers; Asbestos Fibers However, recent scientific studies have thrust asbestos into a negative light and many governmental restrictions have been placed on its use.

High death rates from lung diseases and cancers of the chest and abdomen have been recorded among asbestos workers who did not wear protective gear. Asbestos is also a. However, optical microscopic techniques cannot; [determine fibers of less than approximately 1 ym in diameter, and phase ;contrast cannot differentiate between asbestos and nonasbestos fibers.: The electron microscope (EM) provides particle morphology and size, and a.

sampling - Membrane Filter - Optical Microscopy - Electron Microscopy INTRODUCTION Airborne asbestos fibers in working environments, where the source and type of asbestos fibers are known, are usually analyzed by phase contrast optical microscopy (PCM) in File Size: 3MB.

In patients with diffuse lung diseases, differentiating occupational lung diseases from other diseases is clinically important. However, the value of assessing asbestos and particles in bronchoalveolar lavage fluid (BALF) in diffuse lung diseases by electron microscopy (EM) remains unclear.

We evaluated the utility of EM in detecting asbestos fibers and particles in Cited by: 1. Free Online Library: Asbestos fiber content of lungs with diffuse interstitial fibrosis: an analytical scanning electron microscopic analysis of cases.(Report) by "Archives of Pathology & Laboratory Medicine"; Health, general Asbestosis Diagnosis Research Risk factors Electron microscopy Usage Fibrosis Microscope and microscopy Microscopy Respiratory tract.

Free Online Library: Case report: analytical electron microscopy of lung granulomas associated with exposure to coating materials carried by glass wool fibers.(Research, Case study) by "Environmental Health Perspectives"; Health, general Environmental issues Ceramic materials Ceramics Coatings Health aspects Electron microscopy Usage Granuloma.

Electron microscopy is already in use to aid the diagnosis of mesothelioma, when light microscopy analysis is inconclusive. But the tissue samples needed for this type of analysis need to be of high quality.

In patients with cancer involving the lungs, liquid often accumulates outside the lungs — a feature called pleural effusion. A second, widely-used means of identification of asbestos in material samples is the use of SEM (Scanning Electron Microscopy) or TEM (Transmission Electron Microscopy) - approaches using a directed beam of electrons, an X-ray or EDX (energy-dispersive X-rays) beam, and proper computer software and hardware in support.

ABSTRACTFrom November to Octoberthe French general directorate for labor organized a large field-study using analytical transmission electron microscopy (ATEM) to characterize occupational exposure to asbestos fibers during work on asbestos containing materials (ACM).

The primary objective of this study was to establish a method and to validate Cited by: 2. Fibers were recovered on a membrane filter using a sodium hypochlorite digestion-concentration technique, and the numbers and dimensions of the fibers.

An analytical procedure for the quantitative assessment of asbestos pollution in air, water and biological materials is described. The characterization steps consist of the determination of the total number of fibers in a sample with phase contrast light microscopy and the identification of a significant fraction of those fibers with quantitative X-ray analysis in a Cited by: 1.

Transite - A trade name for asbestos cement wallboard and pipe. Transmission Electron Microscopy - A method of analyzing air samples for asbestos fibers using a transmission electron microscope and, possibly, associated instruments for further identifying asbestos.

Tremolite. - One of six naturally-occurring asbestos minerals. Tremolite has few. Asbestos (pronounced: / æ s ˈ b ɛ s t ə s / or / æ s ˈ b ɛ s t ɒ s /) is a term used to refer to six naturally occurring silicate are composed of long and thin fibrous crystals, each fiber being composed of many microscopic 'fibrils' that can be released into the atmosphere by abrasion and other processes.

Asbestos is an excellent electrical insulator and is highly Category: Silicate minerals. diffuse lung diseases by electron microscopy (EM) remains unclear. We evaluated the utility of EM in detecting asbestos fibers and particles in patients with diffuse lung diseases.

Methods: The BALF specimens of patients with diffuse lung diseases were evaluated. First, detection of asbestos by EM and light microscopy (LM) were by: 1. When electron microscopy was applied to asbestos analysis, hundreds of fibers were discovered present too small to be visible in any light microscope.

There are two different types of electron microscope used for asbestos analysis: Scanning Electron Microscope (SEM) and Transmission Electron Microscope (TEM).File Size: KB. A classification method for asbestos () fibers using scanning electron microscopy (SEM) and computer assisted image digitized sizing was developed and evaluated.

Seven groups of asbestos were tested. An SEM micrograph of a randomly selected field was taken at magnification of X, and referred to as the primary micrograph (PM). The pulmonary toxicity of short fiber asbestos () preparations was studied in rats.

Wistar-rats were injected intratracheally with 40 milligrams (mg) chrysotile (), crocidolite (), tremolite (), or actinolite (), given in. (). Assessment of occupational exposure to asbestos fibers: Contribution of analytical transmission electron microscopy analysis and comparison with phase-contrast microscopy.

Journal of Occupational and Environmental Hygiene: Vol. 15, No. 3, pp. Cited by: 2. Summary. An analytical procedure for the quantitative assessment of asbestos pollution in air, water and biological materials is described.

The characterization steps consist of the determination of the total number of fibers in a sample with phase contrast light microscopy and the identification of a significant fraction of those fibers with quantitative X-ray analysis in a Cited by: 1. These relationships can be expressed mathematically as follows: CROCIDOLITE ASBESTOS CLEARANCE IN RATS 97 Asbestos/rat (txg) = N.

m w [1] = N" p" ld2 w [2] where N = number of asbestos fibers/gram perfusion-fixed rat lung P = density of crocidolite = ~tg/~m3 1 = mean asbestos fiber length in microns d = mean asbestos fiber Cited by: The number of asbestos bodies in iron-stained 5-micrometers histological lung tissue sections was determined by optical microscopy, and the pulmonary concentration of asbestos fibers was.

A Discussion of Asbestos Detection Techniques for Air and Soil CONTENTS INTRODUCTION 1 BACKGROUND 1 ASBESTOS MINERAL TYPES 1 THE HEALTH EFFECTS OF ASBESTOS 3 The Toxicity of Asbestos Fibers 4 Asbestos Regulations 6 Asbestos Counting Methods 7 Phase Contrast Microscopy Counting Method 7 AHERA Counting Method 8 PCM.

Subsequently, there is a progressive decrease in the numbers of fibers seen by scanning electron microscopy at the bifurcations with increasing time from initial exposure. The purpose of the present study was to assess the number and dimensions of fibers deposited in the lungs of these rats and to determine how these parameters changed at Cited by: The methods available for analyzing small lung samples include the classic pathology evaluation by light microscopy used for grading fibrotic involvement and identifying the presence of FBs in tissue sections and the less widely used, but potentially more specific, electron microscopy (EM) analysis.4, 6 Lung tissue digests, in particular,7, 8 Cited by: The detection and counting of asbestos fibers in drinking water samples is an environmental problem which requires the use of an electron microscope.

Since water samples contain pieces of biological and mineral material, a procedure for identifying individual fibers by electron microscopy, electron diffraction and x-ray energy dispersive. The remaining nine cases showed no evidence of an asbestos etiology. Electron microscopic analysis of pulmonary mineral fibers may be required to differentiate asbestos-related mesotheliomas from non-asbestos-related cases when AB counts are within the range of background values.

Polarized Light Microscopy Digital Image Gallery Short Asbestos Fibers. Asbestos is a generic term used to refer to six different types of naturally occurring mineral fibers. The most familiar type of the material and that which is in greatest commercial use. DESIGN: Fiber burden analysis was performed using scanning electron microscopy and energy-dispersive x-ray analysis of lung parenchyma from 86 patients with DPF and patients with asbestosis.

The correlation of the number of asbestos fibers found for a quantitative degree of fibrosis was analyzed. Yes, sorta. But the fibers still do a lot of damage to the body during the decades before they are broken down.

Different types of asbestos have different levels of “biopersistence”. Different types have different resistance to chemicals, and that. • Asbestos fibers do not dissolve in water or evaporate, resistant to heat, fire, chemical and biological degradation o These properties are why asbestos has been mined for a wide range of manufactured products, mostly in building materials and heat resistant fabrics.

On the other hand, electron microscopy can provide a more accurate differentiation between asbestos and non-asbestos fibers, along with the ability to identify the fiber type.

Considering the differences between FM and electron microscopy, an important step in the validation of PCM-FM method is to determine whether PCM-FM counts are equivalent Author: Tomoki Nishimura, Maxym Alexandrov, Takenori Ishida, Ryuichi Hirota, Takeshi Ikeda, Kiyoshi Sekiguch.

Asbestos content was determined by transmission electron microscopy. In exposed subjects, mean fiber concentrations were +/- x 10(6) fibers/g of dry tissue in lung, +/- in black spots, and +/- in normal by:   Using scanning electron microscopy, his lung fibre burden was calculated to be fibres and 31 asbestos bodies per gram of dry weight of lung tissue.

The mean aspect ratio of the anthophyllite fibres in the lung was (SD = ). No other types of asbestos were detected in the lung. His exposure was almost certainly by: 5. Human exposure to asbestos fibers Size distribution of asbestos fibers in the environment In the present paper, we report original data of several analyses requested by ANSES carried out using trans-mission electron microscopy (TEM) [2] (Additional file Cited by: Asbestos is generic term used for any of the 6 types of naturally occurring mineral fibers.

How is the word "asbestos" used in the industry? All asbestos minerals fibers contain silicate with varying proportions of mangnesium, iron, calcium, aluminum, sodium and other trace elements.Scanning Electron Microscopy of Asbestos-Containing Material where the Asbestos Fibers are Considered Locked in a Binder.

There has been a hypothesis that all raw asbestos fibers are encapsulated in solvents and binders and are not released as individual fibers if the material is cut or abraded.

Examination of a number of different types of.